Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 16th Global Diabetes Conference and Medicare Expo Rome, Italy.

Day 2 :

Keynote Forum

Vladimír Štrbák

Slovak Medical University, Slovakia

Keynote: Direction of insulin to regulatory secretory pathway: Possible role in diabetes mellitus

Time : 09:30-10:15

OMICS International Diabetes Global 2017 International Conference Keynote Speaker Vladimír Štrbák photo
Biography:

Vladimír Štrbák has completed his study in Bratislava. He was the Director of Institute of Experimental Endocrinology- SAS, Slovakia, and the Head of Pathological Physiology, Slovak Medical University, Slovakia. He is the President of the Slovak Physiological Society, Council Member FEPS, Chair of the Scientific Board of the Slovak Academy of Sciences for Medical Sciences, and Council Member of the International Society for Pathophysiology. He has 137 published PuBMed papers. He has served as an Organizing Committee Member of the Global Diabetes Summit and Medicare Expo in Dubai (2015) and Birmingham (2016).

Abstract:

High blood insulin concentration at basal conditions without appropriate response to glucose stimulation is common symptom in patients with diabetes mellitus 2 considered to be result of insulinoresistance. Pancreatic thyrotropin-releasing hormone (TRH) is co-localized with insulin in the secretory granules of b cells. We showed that secretion of TRH from islets is stimulated by glucose and inhibited by insulin or somatostatin. Moreover, it was shown that prepro-TRH gene disruption in mice results in hyperglycemia, accompanied by impaired insulin response to glucose stimulation. These data indicate specific relation between TRH and glucose-induced insulin secretion. To induce acute shortage of TRH we blocked the terminal step of the post-translational TRH maturation in adult rat in vivo by disulfiram (DS, 5 day i.p. 200 mg/kg pretreatment) and tested insulin secretion from isolated islets in vitro. Release of insulin from DS-treated pancreatic islets under basal (unstimulated) conditions was four times higher compared to control and could not be further stimulated by high-glucose. Addition of 1 nM TRH in the incubation medium immediately decreased basal insulin secretion to control levels and normalized response to 16.7 mM glucose of islets from DS treated rats. Apparently, pancreatic TRH is necessary for adequate insulin secretion at basal condition and response to glucose stimulation. We conclude that TRH is essential for insulin direction from constitutional to regulatory secretory pathway. This function might be disturbed in diabetes mellitus 2.

Keynote Forum

Nicolette N Houreld

University of Johannesburg, South Africa

Keynote: Mechanisms of action of photobiomodulation in diabetic wound healing

Time : 10:15-11:00

OMICS International Diabetes Global 2017 International Conference Keynote Speaker Nicolette N Houreld photo
Biography:

Nicolette N Houreld has expertise in the areas of photobiomodulation (PBM), otherwise known as low level laser/light therapy (LLLT), and diabetic wound healing. Her research elucidates fundamental biological questions in determining the underlying molecular and cellular mechanisms of action of photobiomodulation which has leads to the promotion and development of highly competitive sustainable research of excellence. Her research has received both national and international acknowledgement for her scientific contribution in the field of Photobiomodulation. She has supervised a number of postgraduate students and sits on various university research committees. She is on the Editorial Board of an international journal, reviews papers for numerous journals, and is on the Executive Committee for an International Medical Society.

Abstract:

Background & Aim: Photobiomodulation (PBM), frequently referred to as low intensity laser/light therapy (LLLT), is a non-invasive, non-thermal treatment modality which induces a photochemical reaction. It involves the application of low power red and near infrared light (NIR) to injuries and lesions with the aim of reducing inflammation and edema and stimulating healing and repair. Diabetes mellitus is a chronic metabolic order which affects many of the bodies systems, and patients are prone to recurring, non-healing, chronic ulcers, particularly in the lower extremities which frequently necessitate amputation. PBM has been found to be beneficial in speeding up the healing of these ulcers and in doing so, improves the quality of life of these patients. However, the exact underlying mechanisms of action are poorly understood. The purpose of this study is to determine the underlying mechanisms of action of PBM in diabetic wounded cells.

Methodology: A diabetic wounded cell model was simulated in WS1 fibroblast cells. Cells were irradiated at 636, 660 (visible red) and 830 nm (NIR) at a fluence of 5 J/cm2. Cellular migration, viability, morphology, collagen production, extracellular matrix (ECM) proteins, enzymes involved in the mitochondrial transport chain, and various genes involved in wound healing were evaluated.

Findings: PBM in the visible red and NIR spectrum stimulated diabetic wounded cells in vitro. There was an increase in cellular migration, proliferation, viability, collagen production, and cytochrome c oxidase activity, as well as an upregulation of COX6B2 and COX6C. A down-regulation in proteases was also observed.

Conclusion: This work has shown that PBM is able to positively stimulate diabetic wounded cells in vitro and leads to healing of such wounds. Photon energy is directly absorbed by the mitochondria stimulating mitochondrial enzymatic activity and leading to the increased production of adenosine triphosphate. This leads to down-stream effects such as increased proliferation, migration and modulation of growth factors and ECM proteins all required for diabetic wound healing.