Ishrat Jahan Saifi
Aligarh Muslim University, India
Title: Impact of glycation on human serum albumin and its role in diabetes mellitus
Biography
Biography: Ishrat Jahan Saifi
Abstract
Diabetes is a metabolic disease; it results due to lack of insulin production, insulin action or both. It became epidemic in all over the world. Glycation of proteins is a major contributor to development of diabetes mellitus. HSA is the abundant protein in human blood; it is highly prone to glycation by the reducing sugars in-vitro and in-vivo in hyperglycemic condition. 2-deoxy D-ribose (dRib) is a reducing sugar having high reactivity with protein and it is present in the human blood. In present study, HSA was incubated with different concentrations of dRib at 37ºC for 4 hours. Structural changes in HSA were determined by UV spectroscopy, fluorescence spectroscopy, circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR). To determine the role of dRib modified HSA in diabetes mellitus, immunological study has been done with the patients sera. We observed preferential binding affinity in the modified HSA coated wells as compare to native HSA coated wells. Thus, it can be concluded that dRib is potent enough to cause structural changes as well as generation of neoepitopes on the protein which may induce antibodies in diabetic patients and might play a role in the onset and progression of diabetes.